ISOLATION OF ADRENAL CORTICAL HORMONES FROM URINE: 17-HYDROXYCORTICOSTERONE AND 17-HYDROXY-11DEHYDROCORTICOSTERONE

Isolation of steroids from the urine of patients with adrenal cortical tumors and adrenal cortical hyperplasia; a new 17-ketosteroid, androstane-3 (alpha) 11-diol-17-one.

Information on the metabolism of the steroid hormones has been obtained largely by injection of known amount,s of the crystalline hormones and isolation of the steroids which subsequently appeared in the urine (1). Experiments of t’his type wit,h the adrenal hormones necessarily have been limited in scope by the limited available supply of crystalline adrenzl steroids. Some additional informati...

Isolation of 17-hydroxycorticosterone from the urine in a case of Cushing's syndrome associated with severe diabetes mellitus.

Many of the features of Cushing’s syndrome are those that would be expected to result from an oversupply of adrenal cortical hormones. Indeed, Albright (1) has attributed the development of this syndrome to overproduction by the adrenal cortex of the carbohydrate-active hormones (S hormone), while Kenyon (2) and Kepler and his associates (3) have explained some of the features of the syndrome, ...

Isolation of i-androstan-6-ol-17-one from urine of patients with adrenal neoplasm.

Partial synthesis of compounds related to adrenal cortical hormones; 3alpha, 11 alpha-dihydroxy-17 alpha-steroids.

In Paper II we (1) have shown that methyl 3(a)-acetoxy-11,12epoxycholanate undergoes fission of the epoxide ring with the introduction of the anionic constituent at C-11. The products of such reactions offered, we felt, a possible route to the preparation of 3(a), 11-dihydroxycholanic acid. Marker and Lawson (2) prepared 3((r), 11-dihydroxy-1Zketocholanic acid by bromination and subsequent alka...

Isolation and characterization of 17 -hydroxy steroid dehydrogenase from human erythrocytes.

1. The 17beta-hydroxy steroid dehydrogenase was solubilized during haemolysis of erythrocytes and was isolated from the membrane-free haemolysate. Membrane preparations isolated in different ways did not contain 17beta-hydroxy steroid dehydrogenase activity. The 17beta-hydroxy steroid dehydrogenase activity in the haemolysate was concentrated by repeated ammonium sulphate precipitation and gel ...